Isolation and antigenic reactivity of Brucella ovis outer membrane proteins
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چکیده
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ISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99
Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...
متن کاملisolation and purification of major outer membrane proteins from brucella abortus s-99
isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...
متن کاملAntigenic relationship of Brucella ovis and Brucella melitensis.
Immune sera were prepared in rabbits by the injection of living and acetone-killed cells of Brucella ovis and smooth and rough B. melitensis. The use of whole-cell antigens in agglutination and agglutinin-absorption tests revealed little relationship between B. ovis and smooth B. melitensis, although there was extensive cross-agglutination between B. ovis and rough B. melitensis. The use of wat...
متن کاملAntigenic relationship of Brucella ovis to Brucella abortus and Brucella melitensis using the complement fixation test.
WORTHINGTON, R. W. & MULDERS, MARIAS. G. Antigenic relationship of Brucellaovis to Brucella abortus and Brucella melitensis using the complement fixation test. Onderstepoort J. vet. Res., 36 (2) , 191-1 98, 1969. The CF test was used to investigate the serological relationship of Br. avis to Br. abortus and Br. melitensis. A definite antigenic relationship between Br. avis and Br. abortus could...
متن کاملRole of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis.
The genes coding for the five outer membrane proteins (OMPs) of the Omp25/Omp31 family expected to be located in the outer membrane (OM) of rough virulent Brucella ovis PA were inactivated to evaluate their role in virulence and OM properties. The OM properties of the mutant strains and of the mutants complemented with the corresponding wild-type genes were analyzed, in comparison with the pare...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1987
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.25.11.2132-2135.1987